Selected article for: "prior infection and viral rna"
Author: de Vries, Erik; Tscherne, Donna M.; Wienholts, Marleen J.; Cobos-Jiménez, Viviana; Scholte, Florine; García-Sastre, Adolfo; Rottier, Peter J. M.; de Haan, Cornelis A. M.
Title: Dissection of the Influenza A Virus Endocytic Routes Reveals Macropinocytosis as an Alternative Entry Pathway
  • Document date: 2011_3_31
    • ID: 05lnj3w0_8
    Snippet: To identify and characterize potential non-CME entry routes taken by IAV, we adapted a luciferase reporter assay [22] to enable the quantitative determination of infection or entry by measuring the activity of secreted Gaussia luciferase. Twentyfour hours prior to infection HeLa cells were transfected with a plasmid (pHH-Gluc) allowing constitutive synthesis (driven by the human PolI promoter) of a negative strand viral RNA (vRNA) encoding a Gaus.....
    Document: To identify and characterize potential non-CME entry routes taken by IAV, we adapted a luciferase reporter assay [22] to enable the quantitative determination of infection or entry by measuring the activity of secreted Gaussia luciferase. Twentyfour hours prior to infection HeLa cells were transfected with a plasmid (pHH-Gluc) allowing constitutive synthesis (driven by the human PolI promoter) of a negative strand viral RNA (vRNA) encoding a Gaussia luciferase under control of the untranslated regions (UTRs) of the NP segment of Influenza A/WSN/33 (H1N1) (hereafter called IAV-WSN) NP segment. Upon IAV infection, the combined expression of the viral polymerase subunits and NP will drive transcription of luciferase mRNA from the negative strand vRNA and subsequent synthesis of Gaussia luciferase.

    Search related documents:
    Co phrase search for related documents
    • assay luciferase reporter and entry route: 1
    • assay luciferase reporter and HeLa cell: 1
    • assay luciferase reporter and IAV infection: 1, 2, 3
    • assay luciferase reporter and luciferase reporter: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25
    • assay luciferase reporter and luciferase transcription: 1, 2, 3
    • entry infection and HeLa cell: 1
    • entry infection and IAV infection: 1, 2, 3, 4, 5, 6
    • entry infection and luciferase reporter: 1, 2, 3, 4
    • entry route and IAV infection: 1, 2
    • entry route and luciferase reporter: 1
    • Gaussia luciferase and IAV infection: 1
    • Gaussia luciferase and luciferase reporter: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10
    • Gaussia luciferase and luciferase transcription: 1
    • HeLa cell and infection HeLa cell: 1, 2, 3
    • HeLa cell and luciferase reporter: 1, 2, 3, 4, 5
    • IAV infection and luciferase reporter: 1, 2, 3, 4, 5, 6