Author: Mordecai, Gideon J; Miller, Kristina M; Di Cicco, Emiliano; Schulze, Angela D; Kaukinen, Karia H; Ming, Tobi J; Li, Shaorong; Tabata, Amy; Teffer, Amy; Patterson, David A; Ferguson, Hugh W; Suttle, Curtis A
Title: Endangered wild salmon infected by newly discovered viruses Document date: 2019_9_3
ID: 010xj69x_21
Snippet: Similarly, a pool of 100 ml (5 tissues contributing 20 ul each) of the aqueous layer was processed for RNA using the MagmaxÀ96 for Microarrays RNA kit (Ambion Inc, Austin, TX, USA) with a Biomek NXP (Beckman-Coulter, Mississauga, ON, Canada) automated liquid-handling instrument, both based on manufacturer's instructions. The quantity of RNA was analysed using spectrophotometer readings and normalised to 62.5 ng/ml with a Biomek NXP (Beckman-Coul.....
Document: Similarly, a pool of 100 ml (5 tissues contributing 20 ul each) of the aqueous layer was processed for RNA using the MagmaxÀ96 for Microarrays RNA kit (Ambion Inc, Austin, TX, USA) with a Biomek NXP (Beckman-Coulter, Mississauga, ON, Canada) automated liquid-handling instrument, both based on manufacturer's instructions. The quantity of RNA was analysed using spectrophotometer readings and normalised to 62.5 ng/ml with a Biomek NXP (Beckman-Coulter, Mississauga, ON, Canada) automated liquid-handling instrument, based on manufacturer's instructions. Mixed tissue RNA (1 mg) was reverse transcribed into cDNA using the superscript VILO master mix kit (Invitrogen, Carlsbad, CA), following the manufacturer's instructions.
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