Author: Elsie Yekwa; Chutima Aphibanthammakit; Xavier Carnec; Bruno Coutard; Caroline Picard; Bruno Canard; Sylvain Baize; François Ferron
Title: Arenaviridae exoribonuclease presents genomic RNA edition capacity Document date: 2019_2_8
ID: fvp45ho6_6
Snippet: A real-time PCR set-up (Bio-rad) was used to monitor the thermal unfolding of the ExoN domain of NP -MOPV or LCMV alone or in the presence of different divalent ions Mn 2+ , Mg 2+ , Ca 2+ and Zn 2+ . Proteins were equilibrated in a buffer containing 20 mM HEPES pH 7.5, 300 mM NaCl, 5% glycerol. All reactions were set up in a final volume of 25 µl in a 96-well plate with total protein concentration of 1,8 mg/ml, 1x SYPRO Orange and incubated with.....
Document: A real-time PCR set-up (Bio-rad) was used to monitor the thermal unfolding of the ExoN domain of NP -MOPV or LCMV alone or in the presence of different divalent ions Mn 2+ , Mg 2+ , Ca 2+ and Zn 2+ . Proteins were equilibrated in a buffer containing 20 mM HEPES pH 7.5, 300 mM NaCl, 5% glycerol. All reactions were set up in a final volume of 25 µl in a 96-well plate with total protein concentration of 1,8 mg/ml, 1x SYPRO Orange and incubated with or without 5 mM of metal ions.
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