Effects of Storage Time on Total Protein and Globulin Concentrations in Bovine Fresh Frozen Plasma Obtained for Transfusion

Proverbio, D.; Spada, E.; Baggiani, L.; Bagnagatti De Giorgi, G.; Roggero, N.; Belloli, A.; Pravettoni, D.; Perego, R.

In dogs and mice plasma proteins appear to be stable during storage when frozen [16, 17] . Previous studies showed that there was no significant change in total protein and globulin fractions, compared with baseline values, in samples of frozen animal plasma stored for up to 7 days [16] [17] [18] . Most of 2 The Scientific World Journal these studies, however, were carried out on plasma obtained from blood, which had been collected using a needle and syringe, and transferred into lithium-heparin tubes. These conditions are quite different from those used for preparation of plasma intended for transfusion purposes which is typically separated from blood collected using a closed system into bags containing citrate-phosphate-dextrose-adenine-1 (CPDA-1) anticoagulant and stored in plastic bags at −20 ∘ C. Furthermore, there is a lack of information about the protein stability of frozen bovine plasma.