Author: Shenglan Shang; Jiaqi Wu; Xiaoli Li; Xin Liu; Pan Li; Chunli Zheng; Yonghua Wang; Songqing Liu; Jiang Zheng; Hong Zhou
Title: Artesunate interacts with Vitamin D receptor to reverse mouse model of sepsis-induced immunosuppression via enhancing autophagy Document date: 2020_2_27
ID: egntml7e_18
Snippet: LPS tolerance is extensively used to simulate the sepsis-induced immunosuppression phase in vitro (Li et al., 2013) . Herein, a cell model of LPS tolerance was established in mouse PMs) and RAW264.7 cells. Briefly, cells were cultured with LPS (0111: B4, Sigma-Aldrich, 5 . CC-BY-NC-ND 4.0 International license author/funder. It is made available under a The copyright holder for this preprint (which was not peer-reviewed) is the . https://doi.org/.....
Document: LPS tolerance is extensively used to simulate the sepsis-induced immunosuppression phase in vitro (Li et al., 2013) . Herein, a cell model of LPS tolerance was established in mouse PMs) and RAW264.7 cells. Briefly, cells were cultured with LPS (0111: B4, Sigma-Aldrich, 5 . CC-BY-NC-ND 4.0 International license author/funder. It is made available under a The copyright holder for this preprint (which was not peer-reviewed) is the . https://doi.org/10.1101/2020.02.26.966143 doi: bioRxiv preprint ng/mL) for 4 h. Then the culture supernatant was replaced followed by the addition of LPS (100 ng/mL) to establish the LPS-tolerance cells model.
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