Author: Xing, Yang; Liqi, Zhu; Jian, Lin; Qinghua, Yu; Qian, Yang
Title: Doxycycline Induces Mitophagy and Suppresses Production of Interferon-ß in IPEC-J2 Cells Document date: 2017_2_1
ID: 1ljye9pj_10
Snippet: Cell viability was determined by 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyl tetrazoliumbromide (MTT) assay. Cells were seeded in 96-well plate at 1000-3000 cells per well overnight. After incubated with DOX either for the indicated concentrations or time period, 10 µl MTT (5 µg/ml MTT in PBS; Sigma) was added to each well and incubated at 37 • C for 2 h and then removed the supernatant. DMSO (Sigma, 100 µl per well) was used to dissolve the.....
Document: Cell viability was determined by 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyl tetrazoliumbromide (MTT) assay. Cells were seeded in 96-well plate at 1000-3000 cells per well overnight. After incubated with DOX either for the indicated concentrations or time period, 10 µl MTT (5 µg/ml MTT in PBS; Sigma) was added to each well and incubated at 37 • C for 2 h and then removed the supernatant. DMSO (Sigma, 100 µl per well) was used to dissolve the cell pellets. After shaking for 10 min, the absorbance was measured at a wavelength of 570 nm. All of the experiments were performed in sextuplicate, and the relative cell viability (%) was expressed as a percentage relative to the untreated control cells.
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