Author: Shenglan Shang; Jiaqi Wu; Xiaoli Li; Xin Liu; Pan Li; Chunli Zheng; Yonghua Wang; Songqing Liu; Jiang Zheng; Hong Zhou
Title: Artesunate interacts with Vitamin D receptor to reverse mouse model of sepsis-induced immunosuppression via enhancing autophagy Document date: 2020_2_27
ID: egntml7e_46
Snippet: Then the proteins were quantified using a BCA kit (Beyotime). Thirty micrograms of each protein sample were separated by 12% SDS-PAGE and transferred to a polyvinylidene difluoride membrane. The membranes were blocked with 5% BSA and incubated with . CC-BY-NC-ND 4.0 International license author/funder. It is made available under a The copyright holder for this preprint (which was not peer-reviewed) is the Chemiluminescence-marked images were deve.....
Document: Then the proteins were quantified using a BCA kit (Beyotime). Thirty micrograms of each protein sample were separated by 12% SDS-PAGE and transferred to a polyvinylidene difluoride membrane. The membranes were blocked with 5% BSA and incubated with . CC-BY-NC-ND 4.0 International license author/funder. It is made available under a The copyright holder for this preprint (which was not peer-reviewed) is the Chemiluminescence-marked images were developed using the Enhanced Chemiluminescent Substrate (PerkinElmer, Nrowalk, CT, USA) with a ChemiDocâ„¢ Touch Imaging System (Bio-Rad, Hercules, CA, USA) and analyzed using the Image Lab packages (Bio-Rad).
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