Effect of cinnamon water extract on monocyte-to-macrophage differentiation and scavenger receptor activity

Kang, Hee; Park, Sung-Hyun; Yun, Jeong-Moon; Nam, Tae-Gyu; Kim, Young-Eun; Kim, Dae-Ok; Kim, Youn Jung

Samples were analyzed by a reversed-phase HPLC system (Shimadzu, Kyoto, Japan) which consisted of an autosampler (SIL-20A), a binary pump (LC-20 AD), and a photodiode array detector (SPD-20A), and was equipped with a Zorbax Eclipse XDB-C18 column (5 μm × 4.6 mm × 250 mm) (Agilent Technologies, CA, USA). Gradient flows for the two-solvent system (solvent A, 0.1% formic acid in water; solvent B, 0.1% formic acid in acetonitrile) were as follows: 95% A/5% B at 0 min, 85% A/15% B at 10 min, 70% A/30% B at 40 min, 50% A/50% B at 70 min, 40% A/60% B at 75 min, 30% A/70% B at 80 min, 95% A/ 5% at 85 min, and 95% A/5% at 90 min. The flow rate of the mobile phase was 0.6 ml/min with an injection volume of 20 μl. Detection was at 254 nm for catechin, epicatechin, coniferyl aldehyde, coumarin, cinnamic acid, and cinnam aldehyde (Sigma, MO, USA), or at 280 nm for cinnamyl alcohol (Sigma). Compounds from CWE were tentatively identified with a spiked input of authentic standards, in addition to the comparison of their retention time and UV-visible spectral patterns.