Artesunate interacts with Vitamin D receptor to reverse mouse model of sepsis-induced immunosuppression via enhancing autophagy

Shenglan Shang; Jiaqi Wu; Xiaoli Li; Xin Liu; Pan Li; Chunli Zheng; Yonghua Wang; Songqing Liu; Jiang Zheng; Hong Zhou

RAW264.7 cells were washed with cold PBS, crosslinked with 1% formaldehyde and stopped by 0.125 M glycine. Then, cells were sonicated in ChIP lysis buffer to generate 600 bp fragments, followed by an overnight incubation with VDR antibody (Abcam, Cambridge, UK) at 4 °C. Obtained DNA was subjected to semiquantitative or quantitative PCR analysis. Table 3 ) were used to amplify the VDR-binding region in the promoter of ATG16L1 genes according to the previous method reported by other laboratories (Wu et al., 2015) . ChIP assay was performed using the Pierce Agarose ChIP kit following the manufacturer's instructions (Thermo Pierce, Rockford, IL, USA) as described before (Bretin et al., 2016) .