Author: Mordecai, Gideon J; Miller, Kristina M; Di Cicco, Emiliano; Schulze, Angela D; Kaukinen, Karia H; Ming, Tobi J; Li, Shaorong; Tabata, Amy; Teffer, Amy; Patterson, David A; Ferguson, Hugh W; Suttle, Curtis A
Title: Endangered wild salmon infected by newly discovered viruses Document date: 2019_9_3
ID: 010xj69x_33
Snippet: For all samples, after reverse transcription, resultant cDNA was combined with the normalised DNA in a ratio of 1:1 and used as the template for the specific target amplification (STA) step. The STA involves a pre-amplification of all primers to be run on a single dynamic array at low concentrations (0.2 mM of each of the primers), and upon completion, excess primers were removed by treating with Exo-SAP-IT (Affymetrix, Santa Clara, CA) according.....
Document: For all samples, after reverse transcription, resultant cDNA was combined with the normalised DNA in a ratio of 1:1 and used as the template for the specific target amplification (STA) step. The STA involves a pre-amplification of all primers to be run on a single dynamic array at low concentrations (0.2 mM of each of the primers), and upon completion, excess primers were removed by treating with Exo-SAP-IT (Affymetrix, Santa Clara, CA) according to manufacturer's instructions and then diluted 1:5 in DNA re-suspension buffer (Teknova, Hollister, CA).
Search related documents:
Co phrase search for related documents- low concentration and reverse transcription: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14
- low concentration and target amplification: 1, 2, 3
Co phrase search for related documents, hyperlinks ordered by date