Selected article for: "final elongation and PCR amplification"
Author: Borkosky, Silvia S.; Whitley, Corinna; Kopp-Schneider, Annette; zur Hausen, Harald; deVilliers, Ethel-Michele
Title: Epstein-Barr Virus Stimulates Torque Teno Virus Replication: A Possible Relationship to Multiple Sclerosis
  • Document date: 2012_2_22
    • ID: 0fl0heq1_24
    Snippet: In order to investigate whether replication of the transfected TTV-HD14b and TTV-HD14c occurred in the tested cell lines, the genomic DNA isolated from all transfected cell lines was further subjected to long-PCR amplification using TaKaRa LA Taq enzyme (TAKARA BIO INC) and back-to-back primers. The specific primers used for each individual TTV type are documented in Table 1 . The reaction was performed as follows: initial denaturation for 1 min .....
    Document: In order to investigate whether replication of the transfected TTV-HD14b and TTV-HD14c occurred in the tested cell lines, the genomic DNA isolated from all transfected cell lines was further subjected to long-PCR amplification using TaKaRa LA Taq enzyme (TAKARA BIO INC) and back-to-back primers. The specific primers used for each individual TTV type are documented in Table 1 . The reaction was performed as follows: initial denaturation for 1 min at 94uC, followed by 30 cycles of 30 s at 94uC, 1 min at 68uC (TTV-HD14b) or 1 min at 65uC (TTV-HD14c) and 4 min at 72uC, followed by final elongation at 72uC for 10 min.

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