Author: Meng, Fandan; Wu, Nai-Huei; Seitz, Maren; Herrler, Georg; Valentin-Weigand, Peter
Title: Efficient suilysin-mediated invasion and apoptosis in porcine respiratory epithelial cells after streptococcal infection under air-liquid interface conditions Document date: 2016_5_27
ID: 0jsc81sy_31
Snippet: Bacterial strains. The virulent S. suis serotype 2 wild-type strain 10 (designated as wt) was kindly provided by H. Smith, Lelystad, NL 54 . The corresponding suilysin-deficient mutant of wt strain 10 (designated as 10Δ sly) and suilysin-deficient unencapsulated mutant of wt strain 10 (designated as 10Δ cpsΔ sly) were constructed as described in previous studies 35, 39 . In all infection experiments, cryo-conserved bacterial stocks were used a.....
Document: Bacterial strains. The virulent S. suis serotype 2 wild-type strain 10 (designated as wt) was kindly provided by H. Smith, Lelystad, NL 54 . The corresponding suilysin-deficient mutant of wt strain 10 (designated as 10Δ sly) and suilysin-deficient unencapsulated mutant of wt strain 10 (designated as 10Δ cpsΔ sly) were constructed as described in previous studies 35, 39 . In all infection experiments, cryo-conserved bacterial stocks were used and prepared as previously reported 37 . Streptococci were grown on Columbia agar supplemented with 7% sheep blood (Oxoid).
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