Selected article for: "apical filter compartment and horizontal shaker"

Author: Meng, Fandan; Wu, Nai-Huei; Seitz, Maren; Herrler, Georg; Valentin-Weigand, Peter
Title: Efficient suilysin-mediated invasion and apoptosis in porcine respiratory epithelial cells after streptococcal infection under air-liquid interface conditions
  • Document date: 2016_5_27
  • ID: 0jsc81sy_41
    Snippet: Supernatants from the apical compartments of infected and mock infected transwell filter were collected before the washing step at 4 hpi. For the determination of cytotoxicity at 24, 48, 72 hpi 70 μ l PBS+ were added to the apical filter compartment and cells were incubated on a horizontal shaker for 5 min to collect the supernatant......
    Document: Supernatants from the apical compartments of infected and mock infected transwell filter were collected before the washing step at 4 hpi. For the determination of cytotoxicity at 24, 48, 72 hpi 70 μ l PBS+ were added to the apical filter compartment and cells were incubated on a horizontal shaker for 5 min to collect the supernatant.

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