Author: Thomas, Joseph T.; Chen, Qi; Gauger, Phillip C.; Giménez-Lirola, Luis G.; Sinha, Avanti; Harmon, Karen M.; Madson, Darin M.; Burrough, Eric R.; Magstadt, Drew R.; Salzbrenner, Holly M.; Welch, Michael W.; Yoon, Kyoung-Jin; Zimmerman, Jeffrey J.; Zhang, Jianqiang
Title: Effect of Porcine Epidemic Diarrhea Virus Infectious Doses on Infection Outcomes in Naïve Conventional Neonatal and Weaned Pigs Document date: 2015_10_6
ID: 1kjk404o_48
Snippet: One objective of this study was to determine the correlation of PEDV PCR Ct values to the infectious titers. A U.S. PEDV prototype strain cell culture isolate USA/IN19338/2013 was 10-fold serially diluted and tested in this study. Since there are variations among laboratories in reporting virus titer units, we determined the infectious titers of the PEDV dilutions using both end-point titration assay and plaque assay. It was found that one TCID 5.....
Document: One objective of this study was to determine the correlation of PEDV PCR Ct values to the infectious titers. A U.S. PEDV prototype strain cell culture isolate USA/IN19338/2013 was 10-fold serially diluted and tested in this study. Since there are variations among laboratories in reporting virus titer units, we determined the infectious titers of the PEDV dilutions using both end-point titration assay and plaque assay. It was found that one TCID 50 /ml is almost equivalent to one PFU/ml for PEDV. Based on a quantitative PEDV N gene-based rRT-PCR we developed, PEDV with an infectious titer of 5 TCID 50 /ml or 5 PFU/ml had a Ct value of approximate 31 corresponding to about 4.8×10 4 genomic copies/ml. An equation of X = 10 (Ct-47.262)/-3.3969 , where X = genomic copies/ml, was used to transform the Ct values into estimated genomic copies of PEDV RNA per ml in samples. However, these results need to be interpreted with caution. First, a PEDV N gene-based primers and probe and reagents from the Path-ID™ Multiplex One-
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