Author: Fongaro, Gislaine; Nascimento, Mariana A do; Rigotto, Caroline; Ritterbusch, Giseli; da Silva, Alessandra D’ A; Esteves, Paulo A; Barardi, Célia R M
Title: Evaluation and molecular characterization of human adenovirus in drinking water supplies: viral integrity and viability assays Document date: 2013_5_28
ID: 19aj551d_33
Snippet: Quantitative PCR was performed as described by Hernroth et al. (2002) [41] . All amplifications were done in a StepOne Plus® Real-Time PCR System (Applied Biosystems). Each sample was analyzed in triplicate. For each plate, four serial dilutions of standard were run in triplicate for each assay and the genome copies (gc) were measured. Ultra-pure water was used as the non-template control for each assay......
Document: Quantitative PCR was performed as described by Hernroth et al. (2002) [41] . All amplifications were done in a StepOne Plus® Real-Time PCR System (Applied Biosystems). Each sample was analyzed in triplicate. For each plate, four serial dilutions of standard were run in triplicate for each assay and the genome copies (gc) were measured. Ultra-pure water was used as the non-template control for each assay.
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