Author: Fongaro, Gislaine; Nascimento, Mariana A do; Rigotto, Caroline; Ritterbusch, Giseli; da Silva, Alessandra D’ A; Esteves, Paulo A; Barardi, Célia R M
Title: Evaluation and molecular characterization of human adenovirus in drinking water supplies: viral integrity and viability assays Document date: 2013_5_28
ID: 19aj551d_4
Snippet: A combination of cell culture and PCR has allowed detection of infectious viruses that grow slowly or fail to produce cytopathic effects (CPE) in cell culture [6] . Integrated cell culture PCR (ICC-PCR) has the benefits of cell culture and PCR and attempts to compensate for several cell culture disadvantages, such as its time-consuming nature and limited detection sensitivity [9] . However, this method has a disadvantage in that detection of nucl.....
Document: A combination of cell culture and PCR has allowed detection of infectious viruses that grow slowly or fail to produce cytopathic effects (CPE) in cell culture [6] . Integrated cell culture PCR (ICC-PCR) has the benefits of cell culture and PCR and attempts to compensate for several cell culture disadvantages, such as its time-consuming nature and limited detection sensitivity [9] . However, this method has a disadvantage in that detection of nucleic acids of inactivated viruses from environmental samples adsorbed onto cell receptors without cell infection can result in false positives [10] . Therefore, it is necessary to confirm infectious viruses by assaying infection of the permissive cells and subsequent transcription of viral mRNA. Thus, the detection of viral mRNA in cell culture indicates the presence of infectious viral particles [11] .
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