Selected article for: "likelihood model and maximum likelihood model"
Author: Fongaro, Gislaine; Nascimento, Mariana A do; Rigotto, Caroline; Ritterbusch, Giseli; da Silva, Alessandra D’ A; Esteves, Paulo A; Barardi, Célia R M
Title: Evaluation and molecular characterization of human adenovirus in drinking water supplies: viral integrity and viability assays
  • Document date: 2013_5_28
    • ID: 19aj551d_49
    Snippet: Sequencing was carried out in a 3130 Genetic Analyzer with the Big Dye Terminator v3.1 Cycle Sequencing Kit (Applied Biosystems) and following the manufacturer's protocol. Each product was sequenced four times in both directions. The quality of DNA sequences was checked and overlapping fragments were assembled using the BioEdit package 7.0.5 [47] , and ContigExpress (InforMax, Inc.). After the alignment, the sequences were compared with the ones .....
    Document: Sequencing was carried out in a 3130 Genetic Analyzer with the Big Dye Terminator v3.1 Cycle Sequencing Kit (Applied Biosystems) and following the manufacturer's protocol. Each product was sequenced four times in both directions. The quality of DNA sequences was checked and overlapping fragments were assembled using the BioEdit package 7.0.5 [47] , and ContigExpress (InforMax, Inc.). After the alignment, the sequences were compared with the ones deposited in GenBank using the BLAST tool [48] and the molecular characterization was conducted with MEGA 5.0 software [49] . The homology analyses (evolutionary history) were inferred by using the Maximum Likelihood method based on the Kimura 2parameter model and calculated using pairwise deletion. Bootstrap was resampled as a test of phylogeny using 500 replications [50] .

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