Selected article for: "real time and reference gene"
Author: Olsvik, Pål A; Lie, Kai K; Jordal, Ann-Elise O; Nilsen, Tom O; Hordvik, Ivar
Title: Evaluation of potential reference genes in real-time RT-PCR studies of Atlantic salmon
  • Document date: 2005_11_17
    • ID: 1r65yam5_16
    Snippet: Our data, based on geNorm calculations, suggest that the Atlantic salmon EF1A genes that have been tested in the present study may be good candidate reference genes. The GAPDH gene seems unsuitable as a reference in quantitative real-time RT-PCR. With regard to the 18S rRNA gene, this must be applied with caution. Tools like the geNorm applet for Microsoft Excel can be useful to help select the most stable genes in various experiments......
    Document: Our data, based on geNorm calculations, suggest that the Atlantic salmon EF1A genes that have been tested in the present study may be good candidate reference genes. The GAPDH gene seems unsuitable as a reference in quantitative real-time RT-PCR. With regard to the 18S rRNA gene, this must be applied with caution. Tools like the geNorm applet for Microsoft Excel can be useful to help select the most stable genes in various experiments.

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