Selected article for: "copper grid and uranyl acetate"
Author: Lee, Yun Ha; Jang, Yo Han; Kim, Young-Seok; Kim, Jinku; Seong, Baik Lin
Title: Evaluation of green tea extract as a safe personal hygiene against viral infections
  • Document date: 2018_1_8
    • ID: 07sn6d9r_11
    Snippet: The size distribution of influenza virus treated with GTE or PBS was measured by dynamic light scattering (DLS). 5 × 10 7 PFU/mL of PR8 virus was treated with 0.1% GTE or PBS for 24 h at 25°C. After the treatment, the virus samples were applied to the Particle size & Zeta potential Anlayzer (ELS-2000ZS, Otsuka Electronics, Japan). The size distribution was measured twice with accumulation time 200 in PBS solvent at 25°C. The morphology of the .....
    Document: The size distribution of influenza virus treated with GTE or PBS was measured by dynamic light scattering (DLS). 5 × 10 7 PFU/mL of PR8 virus was treated with 0.1% GTE or PBS for 24 h at 25°C. After the treatment, the virus samples were applied to the Particle size & Zeta potential Anlayzer (ELS-2000ZS, Otsuka Electronics, Japan). The size distribution was measured twice with accumulation time 200 in PBS solvent at 25°C. The morphology of the virus treated with GTE was analyzed by cryo-electron microscopy (cryo-EM). Purified influenza viruses were loaded onto the Formvar Film 200 copper grid (Electron Microscopy Sciences). After 60 s of sample adsorption, the grid was negative stained with 1% uranyl acetate. Sample vitrification was performed using Vitrobot (FEI), and the vitrified sample was imaged using a CryoTecnai F20 transmission electron microscope (FEI).

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