Evaluation of potential reference genes in real-time RT-PCR studies of Atlantic salmon

Olsvik, Pål A; Lie, Kai K; Jordal, Ann-Elise O; Nilsen, Tom O; Hordvik, Ivar

qRT-PCR analysis of six genes in gills of six smoltifying Atlantic salmon Figure 2 qRT-PCR analysis of six genes in gills of six smoltifying Atlantic salmon. The same data as in Figure 1 , but now normalized against an index of the three best genes (β-actin, EF1A A and EF1A B ) calculated with the geNorm software. The four groups were analyzed with Kruskal-Wallis test, and if significant, the overall p-value is given in the graphs. For β-actin, there were significant differences between the presmolt and the smoltified group (p < 0.05), the presmolt and the desmolt groups (n<0.01) and between the smolt and desmolt groups (p < 0.05). For EF1A A , there was a significant difference between the presmolt and the desmolt groups (p < 0.01). For EF1A B , there was a significant difference between the smolt and the smoltified groups (p < 0.05). An asterisk denotes significant differences between the groups.