Evaluation of the Efficacy of a Recombinant Adenovirus Expressing the Spike Protein of Porcine Epidemic Diarrhea Virus in Pigs

Liu, Xinsheng; Zhao, Donghong; Zhou, Peng; Zhang, Yongguang; Wang, Yonglu

Anti-PEDV-specific IgG and IgA antibody titers of different groups induced by the recombinant virus rAd-PEDV-S were evaluated in pigs following immunizations IM from 0 dpv to 28 dpv by an indirect ELISA based on a PEDV S2 protein. As shown in Figure 2 (a), compared with the Ad-blank and PBS groups, the rAd-PEDV-S group and commercial PEDV inactivated vaccine group exhibited detectable specific IgG antibody titers initially at 7 dpv that rose continuously until 28 dpv (P < 0.05). At 7 dpv, 14 dpv, 21 dpv, and 28 dpv, the specific IgG antibody titers of the rAd-PEDV-S group and the commercial vaccine group were higher than those of the Ad-blank and PBS groups (P < 0.05). Meanwhile, the specific IgG antibody titer of the commercial inactivated vaccine group was slightly higher than that of the rAd-PEDV-S group at 7, 21, and 28 dpv, but there was no significant difference between them (P > 0.05).