Effects of Storage Time on Total Protein and Globulin Concentrations in Bovine Fresh Frozen Plasma Obtained for Transfusion

Proverbio, D.; Spada, E.; Baggiani, L.; Bagnagatti De Giorgi, G.; Roggero, N.; Belloli, A.; Pravettoni, D.; Perego, R.

This prospective study was performed as an internal quality control at the Veterinary Transfusion Unit Blood Bank of University of Milan (REV). Blood was collected from 20 healthy lactating adult Holstein Friesian donors. Before and after blood collection all cows were given a standard physical examination [19] . A total volume of 4 L of blood was collected from each cow. The protocol for this study was approved by the Institutional Ethical Committee for Animal Care at University of Milan (http://www.unimi.it/cataloghi/comitato etico/CE 19dic2012 verbale.pdf). A closed-collection system was used, consisting of sterile human 450 mL blood bags (TERUMO CPDA-1 triple blood bag, GRIFOLS, Italy) containing 20 mL of citrate-phosphate-dextrose-adenine-1 (CPDA-1) anticoagulant, used to collect blood from each cow. From each cow 8 bags of whole blood were collected. Whole blood was collected in a standard fashion from each cow by jugular venipuncture, using a 16-gauge needle attached to a triple-bag closed-collection system on a blood mixer. The closed-collection system consisted of a primary bag containing 63 mL of citrate phosphate-double dextrose solution as anticoagulant, an additive bag that contained 100 mL of additive solution (SAG-Mannitol), and 1 empty satellite bag.