Author: Khattar, Sunil K.; Nayak, Baibaswata; Kim, Shin-Hee; Xiao, Sa; Samal, Sweety; Paldurai, Anandan; Buchholz, Ursula J.; Collins, Peter L.; Samal, Siba K.
Title: Evaluation of the Replication, Pathogenicity, and Immunogenicity of Avian Paramyxovirus (APMV) Serotypes 2, 3, 4, 5, 7, and 9 in Rhesus Macaques Document date: 2013_10_10
ID: 0littefv_19
Snippet: Sera from infected rhesus monkeys were further assayed for APMV-specific antibodies by Western blotting. Partially purified APMV (5 mg per lane) were subjected to 10% SDS-PAGE under reducing conditions and transferred to a nitrocellulose membrane. Membrane strips were incubated with a 1:500 dilution of day 0 and day 28 serum samples from each individual monkey from each group followed by incubation with a 1:5,000 dilution of HRP-conjugated goat a.....
Document: Sera from infected rhesus monkeys were further assayed for APMV-specific antibodies by Western blotting. Partially purified APMV (5 mg per lane) were subjected to 10% SDS-PAGE under reducing conditions and transferred to a nitrocellulose membrane. Membrane strips were incubated with a 1:500 dilution of day 0 and day 28 serum samples from each individual monkey from each group followed by incubation with a 1:5,000 dilution of HRP-conjugated goat anti-human IgG (Fab specific). The hemagglutinin (HN), fusion (F 0 and F 1 ) nucleocapsid (NP), phophoprotein (P) and matrix (M) proteins specific to a particular APMV serotype were detected by a chemiluminescence assay.
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