Selected article for: "enzyme digestion and restriction enzyme digestion"
Author: Liu, Xinsheng; Zhao, Donghong; Zhou, Peng; Zhang, Yongguang; Wang, Yonglu
Title: Evaluation of the Efficacy of a Recombinant Adenovirus Expressing the Spike Protein of Porcine Epidemic Diarrhea Virus in Pigs
  • Document date: 2019_10_8
    • ID: 1lxd2457_10
    Snippet: e complete sequence of the S gene of the PEDV strain CH/ HBXT/2018 was amplified and sequenced. en, restriction enzyme sites for Not I and Afl II were introduced separately at the 5′ and 3′ ends, respectively, of the S gene along with a His-tag and synthesized by Sangon Biotech (Shanghai, China). e synthesized S gene products encoding the S protein and the pDC316-mCMV-EGFP vector (Invitrogen, USA) were digested with Not I and Afl II and then .....
    Document: e complete sequence of the S gene of the PEDV strain CH/ HBXT/2018 was amplified and sequenced. en, restriction enzyme sites for Not I and Afl II were introduced separately at the 5′ and 3′ ends, respectively, of the S gene along with a His-tag and synthesized by Sangon Biotech (Shanghai, China). e synthesized S gene products encoding the S protein and the pDC316-mCMV-EGFP vector (Invitrogen, USA) were digested with Not I and Afl II and then ligated using T4 DNA Ligase (NEB, USA) at 16°C for 1 hour. e ligation products were transformed into TOP10 competent cells (Invitrogen, USA), and positive clones were confirmed by restriction enzyme digestion and sequencing. e correct recombinant transfer bacmid was named "pDC316-S-EGFP."

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