Author: Liu, Xinsheng; Zhao, Donghong; Zhou, Peng; Zhang, Yongguang; Wang, Yonglu
Title: Evaluation of the Efficacy of a Recombinant Adenovirus Expressing the Spike Protein of Porcine Epidemic Diarrhea Virus in Pigs Document date: 2019_10_8
ID: 1lxd2457_17
Snippet: At 28 dpv, to evaluate the efficacy of rAd-PEDV-S, all pigs of each group were challenged orally with 2 mL of cellcultured P4 virus containing 1 × 10 5 TCID 50 highly virulent genotype GIIb PEDV strain CH/HNPJ/2017 [28] . Before the challenge, fecal rectal swabs were collected from all piglets and tested by real-time PCR to confirm PEDV-negative results. After the challenge, clinical signs of PEDV infection were observed daily for 7 days, and cl.....
Document: At 28 dpv, to evaluate the efficacy of rAd-PEDV-S, all pigs of each group were challenged orally with 2 mL of cellcultured P4 virus containing 1 × 10 5 TCID 50 highly virulent genotype GIIb PEDV strain CH/HNPJ/2017 [28] . Before the challenge, fecal rectal swabs were collected from all piglets and tested by real-time PCR to confirm PEDV-negative results. After the challenge, clinical signs of PEDV infection were observed daily for 7 days, and clinical scores of fecal consistency were determined according to the methods of a previous study as follows: 0 � normal, 1 � pasty, 2 � semiliquid, and 3 � liquid [30] . Fecal samples were collected daily to monitor virus shedding in feces during the experiment period.
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