Evaluation of the Efficacy of a Recombinant Adenovirus Expressing the Spike Protein of Porcine Epidemic Diarrhea Virus in Pigs

Liu, Xinsheng; Zhao, Donghong; Zhou, Peng; Zhang, Yongguang; Wang, Yonglu

Similarly, as shown in Figure 2 (b), the specific IgA antibody titers of pigs from the recombinant virus rAd-PEDV-S and commercial inactivated vaccine groups were significantly higher than those of pigs from the Ad-blank and PBS groups from 7 to 28 dpv (P < 0.05), while there was no significant difference between the rAd-PEDV-S and inactivated vaccine groups (P > 0.05). e ability of the recombinant virus rAd-PEDV-S to induce VN antibodies against PEDV was assessed using a VN test. Similar to the levels of serological IgG and IgA antibodies, the results of the VN test revealed that immunization IM with rAd-PEDV-S also elicited VN responses against PEDV in all vaccinated pigs (Figure 2(c) ). Meanwhile, no VN responses were detected in the pigs of the Adblank and PBS groups (Figure 2(c) ). Together, these results indicated that the recombinant virus rAd-PEDV-S could induce a strong humoral immune response in pigs.