Evaluation of the Efficacy of a Recombinant Adenovirus Expressing the Spike Protein of Porcine Epidemic Diarrhea Virus in Pigs

Liu, Xinsheng; Zhao, Donghong; Zhou, Peng; Zhang, Yongguang; Wang, Yonglu

Currently, adenovirus vector systems derived from human adenoviruses have been widely used in the development of new-generation genetically engineered vaccines [25] [26] [27] . ey show more advantages than other vectors, such as easy construction, high efficiency for gene transfer and propagation, high titers and safety, and strong induction of humoral, mucosal, and cellular immune responses [25] . erefore, the recombinant adenovirus vector vaccine is a promising approach for the development of new viral vaccines. However, so far, toward a promising vaccine, the study of a recombinant adenovirus vector vaccine for PEDV is underdeveloped. erefore, the development of new PEDV vaccines based on recombinant adenovirus vectors is needed. To achieve this objective, in this study, a novel recombinant adenovirus expressing the PEDV S protein was generated, and its immunogenicity and protective efficacy were evaluated in 4-week-old pigs.