Author: Andrew C Nelson; Benjamin Auch; Matthew Schomaker; Daryl M Gohl; Patrick Grady; Darrell Johnson; Robyn Kincaid; Kylene E Karnuth; Jerry Daniel; Jessica K Fiege; Elizabeth J Fay; Tyler Bold; Ryan A Langlois; Kenneth B Beckman; Sophia Yohe
Title: Analytical Validation of a COVID-19 qRT-PCR Detection Assay Using a 384-well Format and Three Extraction Methods Document date: 2020_4_5
ID: ec3egn8o_12
Snippet: Three separate 10 µL RT-qPCR reactions were set up in a 384-well Barcoded plate (Thermo Scientific) for either the N1, N2, or RP primers and probes. 2.5 µL extracted RNA was added to 7.5 µL qPCR mastermix comprised of the following components: 1.55 µL water 5 µL GoTaq ® Probe qPCR Master Mix with dUTP (2X) (Promega, Cat # A6120 and A6121) 0.2 µL GoScriptTM RT Mix for 1-Step RT-qPCR (Promega, Cat # A6120 and A6121) 0.75 µL primer/probe set.....
Document: Three separate 10 µL RT-qPCR reactions were set up in a 384-well Barcoded plate (Thermo Scientific) for either the N1, N2, or RP primers and probes. 2.5 µL extracted RNA was added to 7.5 µL qPCR mastermix comprised of the following components: 1.55 µL water 5 µL GoTaq ® Probe qPCR Master Mix with dUTP (2X) (Promega, Cat # A6120 and A6121) 0.2 µL GoScriptTM RT Mix for 1-Step RT-qPCR (Promega, Cat # A6120 and A6121) 0.75 µL primer/probe sets for either N1, N2, or RP (IDT, Cat# 10006713)
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