Evaluation of the Efficacy of a Recombinant Adenovirus Expressing the Spike Protein of Porcine Epidemic Diarrhea Virus in Pigs

Liu, Xinsheng; Zhao, Donghong; Zhou, Peng; Zhang, Yongguang; Wang, Yonglu

e highly virulent Chinese genotype GIIa PEDV strain CH/HBXT/2018 (GenBank accession number MH816969) was isolated by our laboratory and propagated in Vero cells (ATCC CCL-81) as previously described [28] . e Ad5Max adenovirus vector system derived from human adenovirus serotype 5 was obtained from GeneCreate Biological Engineering Company (Wuhan, China). e Vero cells were grown in Dulbecco's modified Eagle's medium (DMEM; Invitrogen, USA) containing 10% heat-inactivated fetal bovine serum (FBS; Invitrogen, Australia) and 1% antibiotics and antimycotics (10,000 units of penicillin, 10,000 μg of streptomycin, and 25 μg of Fungizone ® per milliliter) (Gibco ™ , USA) and cultured at 37°C with 5% CO 2 . HEK293A cells were obtained from GeneCreate Biological Engineering Company (Wuhan, China) and were cultured in DMEM (Invitrogen, USA) with 10% heat-inactivated FBS (Gibco ™ , USA).