Author: Meng, Fandan; Ren, Yudong; Suo, Siqingaowa; Sun, Xuejiao; Li, Xunliang; Li, Pengchong; Yang, Wei; Li, Guangxing; Li, Lu; Schwegmann-Wessels, Christel; Herrler, Georg; Ren, Xiaofeng
Title: Evaluation on the Efficacy and Immunogenicity of Recombinant DNA Plasmids Expressing Spike Genes from Porcine Transmissible Gastroenteritis Virus and Porcine Epidemic Diarrhea Virus Document date: 2013_3_19
ID: 081o2zmd_23
Snippet: Serum IL-4 levels were similarly analyzed using an IL-4 detection kit (Excell Bio. China). Control IL-4 was serially-diluted two-fold in PBS between 500 pg/ml and 7.8 pg/ml then coated onto ELISA plates at 37uC overnight. The ELISA was performed as above and OD 490 values (pg/ml) were determined relative to an IL-4 standard curve......
Document: Serum IL-4 levels were similarly analyzed using an IL-4 detection kit (Excell Bio. China). Control IL-4 was serially-diluted two-fold in PBS between 500 pg/ml and 7.8 pg/ml then coated onto ELISA plates at 37uC overnight. The ELISA was performed as above and OD 490 values (pg/ml) were determined relative to an IL-4 standard curve.
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