Author: Meng, Fandan; Ren, Yudong; Suo, Siqingaowa; Sun, Xuejiao; Li, Xunliang; Li, Pengchong; Yang, Wei; Li, Guangxing; Li, Lu; Schwegmann-Wessels, Christel; Herrler, Georg; Ren, Xiaofeng
Title: Evaluation on the Efficacy and Immunogenicity of Recombinant DNA Plasmids Expressing Spike Genes from Porcine Transmissible Gastroenteritis Virus and Porcine Epidemic Diarrhea Virus Document date: 2013_3_19
ID: 081o2zmd_15
Snippet: Peripheral blood was collected by orbital bleeds at 3 h and then at 7, 14, 21, 28, 35 and 42 days post-immunization (dpi); serum and T lymphocytes were subsequently prepared. For detecting TGEV and PEDV specific antibodies, recombinant TGEV-S1 and PEDV-S1 protein purified from pGEX-(TGEV-S1) and pET-(PEDV-S1) transformed bacterial cells was diluted to 50 mg/ml in 0.05 M NaHCO 3 . An ELISA was performed according to published protocols with modifi.....
Document: Peripheral blood was collected by orbital bleeds at 3 h and then at 7, 14, 21, 28, 35 and 42 days post-immunization (dpi); serum and T lymphocytes were subsequently prepared. For detecting TGEV and PEDV specific antibodies, recombinant TGEV-S1 and PEDV-S1 protein purified from pGEX-(TGEV-S1) and pET-(PEDV-S1) transformed bacterial cells was diluted to 50 mg/ml in 0.05 M NaHCO 3 . An ELISA was performed according to published protocols with modifications [22, 24] . To assess antibody binding, ELISA wells were incubated with o-phenylenediamine dihydrochloride substrate for 15 min. The reactions were terminated with 50 ml 2M H 2 SO 4 and the wells read at 490 nm.
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