Author: Meng, Fandan; Wu, Nai-Huei; Seitz, Maren; Herrler, Georg; Valentin-Weigand, Peter
Title: Efficient suilysin-mediated invasion and apoptosis in porcine respiratory epithelial cells after streptococcal infection under air-liquid interface conditions Document date: 2016_5_27
ID: 0jsc81sy_39
Snippet: To determine bacterial replication and growth kinetics, supernatants from the apical compartment of infected transwell filters were collected before the washing step (4 hpi) and plated on Columbia agar supplemented with 7% sheep blood. At the later time points (24, 48 , and 72 hpi) 70 μ l PBS+ was added to the apical filter compartment and cells were incubated on a horizontal shaker for 5 min to collect non-adherent streptococci. Supernatants we.....
Document: To determine bacterial replication and growth kinetics, supernatants from the apical compartment of infected transwell filters were collected before the washing step (4 hpi) and plated on Columbia agar supplemented with 7% sheep blood. At the later time points (24, 48 , and 72 hpi) 70 μ l PBS+ was added to the apical filter compartment and cells were incubated on a horizontal shaker for 5 min to collect non-adherent streptococci. Supernatants were plated as described above.
Search related documents:
Co phrase search for related documents- apical compartment and infected transwell filter: 1
- apical filter and horizontal shaker: 1
- apical filter and infected transwell filter: 1
- apical filter compartment and horizontal shaker: 1
- apical filter compartment and infected transwell filter: 1
- bacterial replication and growth bacterial replication: 1, 2, 3, 4, 5, 6
- Columbia agar and sheep blood: 1, 2, 3, 4, 5
- horizontal shaker and infected transwell filter: 1
Co phrase search for related documents, hyperlinks ordered by date