Endangered wild salmon infected by newly discovered viruses

Mordecai, Gideon J; Miller, Kristina M; Di Cicco, Emiliano; Schulze, Angela D; Kaukinen, Karia H; Ming, Tobi J; Li, Shaorong; Tabata, Amy; Teffer, Amy; Patterson, David A; Ferguson, Hugh W; Suttle, Curtis A

Controls were added prior to running the dynamic array (Miller et al., 2016) . Note, APC clones to all assays were contained in a single serially diluted pool, loaded last, minimising the likelihood of contamination of any single APC clone. Once loading and mixing of the dynamic array was completed within the IFC HX controller, the array was transferred to the BioMark HD instrument and processed using the GE 96 Â 96 Standard TaqMan program for qPCR which includes a hot start followed by 40 cycles at 95˚C for 15 s and 60˚C for 1 min (Fluidigm Corporation, CA, USA). The data were analysed with Real-Time PCR Analysis Software (Fluidigm Corporation, CA, USA).