Evolution of the Bovine TLR Gene Family and Member Associations with Mycobacterium avium Subspecies paratuberculosis Infection

Fisher, Colleen A.; Bhattarai, Eric K.; Osterstock, Jason B.; Dowd, Scot E.; Seabury, Paul M.; Vikram, Meenu; Whitlock, Robert H.; Schukken, Ynte H.; Schnabel, Robert D.; Taylor, Jeremy F.; Womack, James E.; Seabury, Christopher M.

A regression-based approach considering all validated variable sites and the effective number of SNPs at each site [30] also demonstrated that TLR3 and TLR8 possess significantly more gene diversity than do the eight other TLR loci (P#0.05; Figure 5 ) in taurine and all cattle combined. In contrast, both regression analyses (all cattle; B. t. taurus only) indicated that TLR10 and TLR2 possess significantly less gene diversity than other members of the bovine TLR gene family ( Figure 5 ). With the exception of Table S2 for SNP information). Node sizes are proportional to haplotype frequency, and all branch lengths are drawn to scale. Alphabetized letters at nodes represent the breed distribution of each haplotype (Table S4) . Median vectors are indicated as ''mv''. doi:10.1371/journal.pone.0027744.g002 Table S2 for SNP information). Node sizes are proportional to haplotype frequency, and all branch lengths are drawn to scale. Alphabetized letters at nodes represent the breed distribution of each haplotype (Table S4) . doi:10.1371/journal.pone.0027744.g003 TLR2, these results are precisely congruent with the results of Tajima's test (D; Table 4 ).