Author: Meng, Fandan; Ren, Yudong; Suo, Siqingaowa; Sun, Xuejiao; Li, Xunliang; Li, Pengchong; Yang, Wei; Li, Guangxing; Li, Lu; Schwegmann-Wessels, Christel; Herrler, Georg; Ren, Xiaofeng
Title: Evaluation on the Efficacy and Immunogenicity of Recombinant DNA Plasmids Expressing Spike Genes from Porcine Transmissible Gastroenteritis Virus and Porcine Epidemic Diarrhea Virus Document date: 2013_3_19
ID: 081o2zmd_9
Snippet: TGEV S1 and PEDV S1 proteins were expressed in E.coli, respectively for lymphocyte stimulation assays. In brief, two recombinant plasmids pGEX-S1 comprising the 59-end half of the TGEV S gene [26] and pET30a-S1 comprising 59-end of PEDV S gene (97 bp-1776 bp) were constructed and transformed into E.coli BL21 (DE3) pLysS (Novagen, Germany). Expression and purification of TGEV S1 protein were detailed in a recent reference [26] and PEDV S1 protein .....
Document: TGEV S1 and PEDV S1 proteins were expressed in E.coli, respectively for lymphocyte stimulation assays. In brief, two recombinant plasmids pGEX-S1 comprising the 59-end half of the TGEV S gene [26] and pET30a-S1 comprising 59-end of PEDV S gene (97 bp-1776 bp) were constructed and transformed into E.coli BL21 (DE3) pLysS (Novagen, Germany). Expression and purification of TGEV S1 protein were detailed in a recent reference [26] and PEDV S1 protein was also obtained according to a similar protocol. The expressed fused proteins were named TGEV-S1and PEDV-S1, respectively,
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