Selected article for: "gene sequencing and RT PCR"
Author: Drexler, Jan Felix; Corman, Victor Max; Müller, Marcel Alexander; Lukashev, Alexander N.; Gmyl, Anatoly; Coutard, Bruno; Adam, Alexander; Ritz, Daniel; Leijten, Lonneke M.; van Riel, Debby; Kallies, Rene; Klose, Stefan M.; Gloza-Rausch, Florian; Binger, Tabea; Annan, Augustina; Adu-Sarkodie, Yaw; Oppong, Samuel; Bourgarel, Mathieu; Rupp, Daniel; Hoffmann, Bernd; Schlegel, Mathias; Kümmerer, Beate M.; Krüger, Detlev H.; Schmidt-Chanasit, Jonas; Setién, Alvaro Aguilar; Cottontail, Veronika M.; Hemachudha, Thiravat; Wacharapluesadee, Supaporn; Osterrieder, Klaus; Bartenschlager, Ralf; Matthee, Sonja; Beer, Martin; Kuiken, Thijs; Reusken, Chantal; Leroy, Eric M.; Ulrich, Rainer G.; Drosten, Christian
Title: Evidence for Novel Hepaciviruses in Rodents
  • Document date: 2013_6_20
    • ID: 1v353uij_36
    Snippet: For the molecular analysis of bats, 2,939 sera from Gabon, Ghana, Papua-New Guinea, Australia, Thailand, Panama and Germany were tested for Hepacivirus RNA using several broadly reactive and highly sensitive RT-PCR assays, as detailed in Supplementary Table 2 . Despite the apparent relatedness of putative bat hepaciviruses with HCV suggested by the serologic analyses, no hepacivirus RNA was detected in any of the specimens, whereas several PCR fr.....
    Document: For the molecular analysis of bats, 2,939 sera from Gabon, Ghana, Papua-New Guinea, Australia, Thailand, Panama and Germany were tested for Hepacivirus RNA using several broadly reactive and highly sensitive RT-PCR assays, as detailed in Supplementary Table 2 . Despite the apparent relatedness of putative bat hepaciviruses with HCV suggested by the serologic analyses, no hepacivirus RNA was detected in any of the specimens, whereas several PCR fragments from the NS3 gene were obtained which upon sequencing were identified as pegiviruses related to GBV-D [48] .

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