Author: Borkosky, Silvia S.; Whitley, Corinna; Kopp-Schneider, Annette; zur Hausen, Harald; deVilliers, Ethel-Michele
Title: Epstein-Barr Virus Stimulates Torque Teno Virus Replication: A Possible Relationship to Multiple Sclerosis Document date: 2012_2_22
ID: 0fl0heq1_10
Snippet: Having demonstrated virus replication after transfection of TTV-HD14b-and TTV-HD14c-full-length genomes into EBV-positive and -negative cell lines, we quantified the level of TTV replication by real-time PCR. Viral DNA was normalized against the housekeeping gene HMBS and relative quantification was calculated using the EBV-negative cell line BJAB as calibrator. All measurements were performed for each time point when cells were harvested. We use.....
Document: Having demonstrated virus replication after transfection of TTV-HD14b-and TTV-HD14c-full-length genomes into EBV-positive and -negative cell lines, we quantified the level of TTV replication by real-time PCR. Viral DNA was normalized against the housekeeping gene HMBS and relative quantification was calculated using the EBV-negative cell line BJAB as calibrator. All measurements were performed for each time point when cells were harvested. We used two independent primer sets per isolate for the detection of both TTV-HD14b and TTV-HD14c. Two isolates of TTV-HD14 were included in this study in order to avoid possible variations which may result from differences in the TTV genome organization. Primers and probes are listed in Table 1 .
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