Artesunate interacts with Vitamin D receptor to reverse mouse model of sepsis-induced immunosuppression via enhancing autophagy

Shenglan Shang; Jiaqi Wu; Xiaoli Li; Xin Liu; Pan Li; Chunli Zheng; Yonghua Wang; Songqing Liu; Jiang Zheng; Hong Zhou

For the cell samples, the culture supernatants were collected. For blood samples, the serum was collected. For mouse tissue samples, one gram of lungs and spleen tissue was homogenized then the supernatants were collected. The cytokine levels of TNF-α, IL-6, and . CC-BY-NC-ND 4.0 International license author/funder. It is made available under a The copyright holder for this preprint (which was not peer-reviewed) is the . https://doi.org/10.1101/2020.02.26.966143 doi: bioRxiv preprint IL-1β in all of samples were determined using the appropriate ELISA kits (Thermo Fisher Scientific, Waltham, MA, USA), according to the manufactures' protocols. For NF-κB p65 level in the nucleus, the nucleoproteins were extracted and detected using the Trans-AM NF-κB kits (Active Motif, Carlsbad, CA, USA).