Evaluation of the efficacy of a trivalent vaccine mixture against a triple challenge with Mycoplasma hyopneumoniae, PCV2, and PRRSV and the efficacy comparison of the respective monovalent vaccines against a single challenge

Oh, Taehwan; Park, Kee Hwan; Yang, Siyeon; Jeong, Jiwoon; Kang, Ikjae; Park, Changhoon; Chae, Chanhee

At − 35 days post challenge (dpc, 21 days old), pigs in the Vac3FLEX/Ch3 group were administered one dose (2 mL) of 3FLEX (Boehringer Ingelheim Vetmedica) on the left side of the neck. The 3FLEX vaccine was prepared as follows: MycoFLEX (Serial no. 2730534A) and CircoFLEX (Serial no. 3091124A) were mixed together, and the mixture was then used to rehydrate Ingelvac PRRS MLV (Serial no. 2451180A). This was done in place of the Ingelvac PRRS MLV accompanying vaccine diluent and against the manufacturer's mixing directions. Pigs in the VacMhp/ChMhp, VacPCV2/ ChPCV2, and VacPRRS/ChPRRSV groups were administered one dose of MycoFLEX (1 mL), CircoFLEX (1 mL) and Ingelvac PRRS MLV (2 mL) respectively, on the left side of the neck. The pigs in the UnVac/Ch3, UnVac/ChMhp, UnVac/ChPCV2, UnVac/ChPRRSV, and UnVac/UnCh groups were administered one dose (1 mL) of phosphate buffered saline (PBS, 0.01 M, pH 7.4) as a control.