Author: Meng, Fandan; Ren, Yudong; Suo, Siqingaowa; Sun, Xuejiao; Li, Xunliang; Li, Pengchong; Yang, Wei; Li, Guangxing; Li, Lu; Schwegmann-Wessels, Christel; Herrler, Georg; Ren, Xiaofeng
Title: Evaluation on the Efficacy and Immunogenicity of Recombinant DNA Plasmids Expressing Spike Genes from Porcine Transmissible Gastroenteritis Virus and Porcine Epidemic Diarrhea Virus Document date: 2013_3_19
ID: 081o2zmd_20
Snippet: To determine if mice generated TGEV and PEDV neutralizing antibodies, sera (1:20-1:320 dilution, 300 ml) from DNA-vaccinated mice were mixed with an equal volume of PEDV or TGEV (10 5 pfu/ml) at 37uC. After 1 h incubation, the treated viruses were used to infect cultured Swine testis (ST) Cells and African green monkey kidney (Vero) cells in 24-well plates respectively followed by overlaying with 1% methylcellulose. The plates were incubated at 3.....
Document: To determine if mice generated TGEV and PEDV neutralizing antibodies, sera (1:20-1:320 dilution, 300 ml) from DNA-vaccinated mice were mixed with an equal volume of PEDV or TGEV (10 5 pfu/ml) at 37uC. After 1 h incubation, the treated viruses were used to infect cultured Swine testis (ST) Cells and African green monkey kidney (Vero) cells in 24-well plates respectively followed by overlaying with 1% methylcellulose. The plates were incubated at 37uC in a 5% CO 2 atmosphere and examined daily for 3 days for TGEV and PEDV specific cytopathic effects (CPE).
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