Selected article for: "fetal bovine serum and Hyclone 10 FBS fetal bovine serum"

Author: Shenglan Shang; Jiaqi Wu; Xiaoli Li; Xin Liu; Pan Li; Chunli Zheng; Yonghua Wang; Songqing Liu; Jiang Zheng; Hong Zhou
Title: Artesunate interacts with Vitamin D receptor to reverse mouse model of sepsis-induced immunosuppression via enhancing autophagy
  • Document date: 2020_2_27
  • ID: egntml7e_9
    Snippet: The murine macrophage-like cell line RAW264.7 cells and Human monocyte THP-1 cells were purchased from American Type Culture Collection (Manassas, VA, USA). RAW264.7 cells were cultured in Dulbecco's modified Eagle's medium (DMEM) supplemented with 10% low-LPS fetal bovine serum (FBS) (Hyclone, Logan, UT, USA), 1% penicillin/streptomycin (regular medium) in a 37°C humid atmosphere with 5% CO 2 . THP-1 cells were cultured in RPMI 1640 (Hyclone) r.....
    Document: The murine macrophage-like cell line RAW264.7 cells and Human monocyte THP-1 cells were purchased from American Type Culture Collection (Manassas, VA, USA). RAW264.7 cells were cultured in Dulbecco's modified Eagle's medium (DMEM) supplemented with 10% low-LPS fetal bovine serum (FBS) (Hyclone, Logan, UT, USA), 1% penicillin/streptomycin (regular medium) in a 37°C humid atmosphere with 5% CO 2 . THP-1 cells were cultured in RPMI 1640 (Hyclone) regular medium in suspension. THP-1 derived macrophages were obtained by differentiating THP-1 cells with phorbol 12-myristate 13-acetate (PMA) (Sigma-Aldrich, St. Louis, MO, USA) treatment at 100 nM for 24 h according to the previous study .

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