Selected article for: "antibody incubation and primary antibody incubation"
Author: Drelich, Aleksandra; Judy, Barbara; He, Xi; Chang, Qing; Yu, Shangyi; Li, Xiang; Lu, Fanglin; Wakamiya, Maki; Popov, Vsevolod; Zhou, Jia; Ksiazek, Thomas; Gong, Bin
Title: Exchange Protein Directly Activated by cAMP Modulates Ebola Virus Uptake into Vascular Endothelial Cells
  • Document date: 2018_10_16
    • ID: 02xjq8vx_1
    Snippet: For immunoblotting assays, equal amounts of soluble protein were subjected to 12% SDS-polyacrylamide gel electrophoresis (SDS-PAGE). Proteins were transferred onto a polyvinylidene difluoride membrane and then incubated with primary antibody at 1:1000 overnight, followed by incubation with a horseradish peroxidase (HRP)-conjugated secondary antibody at 1:2000 for 1 h. Blots were visualized by using an enhanced chemiluminescence kit (LI-COR Biosci.....
    Document: For immunoblotting assays, equal amounts of soluble protein were subjected to 12% SDS-polyacrylamide gel electrophoresis (SDS-PAGE). Proteins were transferred onto a polyvinylidene difluoride membrane and then incubated with primary antibody at 1:1000 overnight, followed by incubation with a horseradish peroxidase (HRP)-conjugated secondary antibody at 1:2000 for 1 h. Blots were visualized by using an enhanced chemiluminescence kit (LI-COR Biosciences, Lincoln, NE). All experiments were repeated three times.

    Search related documents:
    Co phrase search for related documents
    • chemiluminescence kit and horseradish peroxidase: 1, 2, 3
    • chemiluminescence kit and sds polyacrylamide: 1
    • chemiluminescence kit and secondary antibody: 1, 2
    • equal amount and horseradish peroxidase: 1
    • equal amount and primary antibody: 1
    • equal amount and sds polyacrylamide: 1, 2, 3
    • equal amount and secondary antibody: 1
    • gel electrophoresis and horseradish peroxidase: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10
    • gel electrophoresis and polyvinylidene difluoride: 1, 2, 3, 4, 5
    • gel electrophoresis and polyvinylidene difluoride membrane: 1, 2, 3
    • gel electrophoresis and primary antibody: 1, 2, 3, 4
    • gel electrophoresis and SDS PAGE gel electrophoresis: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25
    • gel electrophoresis and sds polyacrylamide: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25
    • gel electrophoresis and sds polyacrylamide SDS PAGE gel electrophoresis: 1, 2, 3, 4, 5
    • gel electrophoresis and secondary antibody: 1, 2, 3, 4, 5, 6
    • gel electrophoresis and soluble protein: 1, 2, 3, 4
    • horseradish peroxidase and sds polyacrylamide: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13
    • horseradish peroxidase and secondary antibody: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25
    • horseradish peroxidase and soluble protein: 1