Selected article for: "overnight incubation and secondary antibody"
Author: Drelich, Aleksandra; Judy, Barbara; He, Xi; Chang, Qing; Yu, Shangyi; Li, Xiang; Lu, Fanglin; Wakamiya, Maki; Popov, Vsevolod; Zhou, Jia; Ksiazek, Thomas; Gong, Bin
Title: Exchange Protein Directly Activated by cAMP Modulates Ebola Virus Uptake into Vascular Endothelial Cells
  • Document date: 2018_10_16
    • ID: 02xjq8vx_1
    Snippet: For immunoblotting assays, equal amounts of soluble protein were subjected to 12% SDS-polyacrylamide gel electrophoresis (SDS-PAGE). Proteins were transferred onto a polyvinylidene difluoride membrane and then incubated with primary antibody at 1:1000 overnight, followed by incubation with a horseradish peroxidase (HRP)-conjugated secondary antibody at 1:2000 for 1 h. Blots were visualized by using an enhanced chemiluminescence kit (LI-COR Biosci.....
    Document: For immunoblotting assays, equal amounts of soluble protein were subjected to 12% SDS-polyacrylamide gel electrophoresis (SDS-PAGE). Proteins were transferred onto a polyvinylidene difluoride membrane and then incubated with primary antibody at 1:1000 overnight, followed by incubation with a horseradish peroxidase (HRP)-conjugated secondary antibody at 1:2000 for 1 h. Blots were visualized by using an enhanced chemiluminescence kit (LI-COR Biosciences, Lincoln, NE). All experiments were repeated three times.

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