Author: Peters, Rebecca Marie; Schnee, Sarah Valerie; Tabatabai, Julia; Schnitzler, Paul; Pfeil, Johannes
Title: Evaluation of Alere i RSV for Rapid Detection of Respiratory Syncytial Virus in Children Hospitalized with Acute Respiratory Tract Infection Document date: 2017_3_24
ID: 1v56m2vq_8
Snippet: The Alere i RSV test includes a 3-min step of preheating the lysis buffer, followed by the nicking enzyme amplification reaction with a maximum duration of 10 min. The assay identifies positive test results as soon as an amplification threshold is reached. In this study, the median duration of the nicking enzyme amplification reaction in RSV-positive samples was 1.8 min (range, 1.7 min to 3.9 min). Hence, in RSV-negative samples, the total durati.....
Document: The Alere i RSV test includes a 3-min step of preheating the lysis buffer, followed by the nicking enzyme amplification reaction with a maximum duration of 10 min. The assay identifies positive test results as soon as an amplification threshold is reached. In this study, the median duration of the nicking enzyme amplification reaction in RSV-positive samples was 1.8 min (range, 1.7 min to 3.9 min). Hence, in RSV-negative samples, the total duration of the test assay was 13 min (3 min of preheating plus 10 min of amplification), whereas it was only approximately 5 to 7 min in RSV-positive samples.
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