Selected article for: "Atlantic salmon and genorm software"
Author: Olsvik, Pål A; Lie, Kai K; Jordal, Ann-Elise O; Nilsen, Tom O; Hordvik, Ivar
Title: Evaluation of potential reference genes in real-time RT-PCR studies of Atlantic salmon
  • Document date: 2005_11_17
    • ID: 1r65yam5_11
    Snippet: We see a correlation between the A260/230 absorbance on the NanoDrop and the PCR efficiency (data not shown). We tend to get PCR efficiencies that are too high in some samples with low A260/230 ratios. When the samples are treated with DNase solution, the A260/230 ratio usually drops. After DNase treatment, the A260/280 ratio increased from 1.8 to 2.1 (n = 45 samples). At the Table 3 : Evaluation of the usefulness of six potential reference genes.....
    Document: We see a correlation between the A260/230 absorbance on the NanoDrop and the PCR efficiency (data not shown). We tend to get PCR efficiencies that are too high in some samples with low A260/230 ratios. When the samples are treated with DNase solution, the A260/230 ratio usually drops. After DNase treatment, the A260/280 ratio increased from 1.8 to 2.1 (n = 45 samples). At the Table 3 : Evaluation of the usefulness of six potential reference genes in eight tissues of Atlantic salmon ranked by the geNorm software. 1 = best, 6 = worst. Six individuals were analyzed for six genes in eight tissues. qRT-PCR analysis of six genes in gills of six Atlantic salmon going through smoltification; presmolt (before smoltification), smolt (during smoltification), smoltified (finished smoltified in seawater) and desmolt (desmoltification in freshwater) Figure 1 qRT-PCR analysis of six genes in gills of six Atlantic salmon going through smoltification; presmolt (before smoltification), smolt (during smoltification), smoltified (finished smoltified in seawater) and desmolt (desmoltification in freshwater). Numbers indicate raw Ct values.

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