Author: Barnard, Karen N.; Wasik, Brian R.; LaClair, Justin R.; Buchholz, David W.; Weichert, Wendy S.; Alford-Lawrence, Brynn K.; Aguilar, Hector C.; Parrish, Colin R.
Title: Expression of 9-O- and 7,9-O-Acetyl Modified Sialic Acid in Cells and Their Effects on Influenza Viruses Document date: 2019_12_3
ID: 11ejfiwe_31
Snippet: Characterization of A549 conditioned media. Conditioned medium from A549 cells was prepared by washing a fully confluent flask of cells to remove any serum and allowing the cells to grow in serum-free medium for 5 to 7 days. The conditioned medium was collected, dialyzed with 3 volumes of PBS, and concentrated using a 30-kDa centrifugal filter (Pall Corporation). The protein concentration was determined by using a Qubit 4 fluorometer (Invitrogen).....
Document: Characterization of A549 conditioned media. Conditioned medium from A549 cells was prepared by washing a fully confluent flask of cells to remove any serum and allowing the cells to grow in serum-free medium for 5 to 7 days. The conditioned medium was collected, dialyzed with 3 volumes of PBS, and concentrated using a 30-kDa centrifugal filter (Pall Corporation). The protein concentration was determined by using a Qubit 4 fluorometer (Invitrogen). To determine Muc5B presence, 2-fold dilutions of conditioned media were compared to purified human Muc5B using a 8% SDS-PAGE gel and probed with an anti-human Muc5B antibody (both purified protein and antibody were gifts from Stefan Ruhl, University of Buffalo). Conditioned medium was analyzed for total Sia using the HPLC methods listed above.
Search related documents:
Co phrase search for related documents- centrifugal filter and purify protein: 1
- centrifugal filter and SDS PAGE gel: 1, 2
- condition medium and serum free medium: 1, 2
- protein concentration and SDS PAGE gel: 1, 2, 3, 4, 5, 6, 7, 8
- protein concentration and serum free medium: 1
Co phrase search for related documents, hyperlinks ordered by date