Author: Frenzel, André; Hust, Michael; Schirrmann, Thomas
Title: Expression of Recombinant Antibodies Document date: 2013_7_29
ID: 06o7pa3d_12
Snippet: The Fab format requires expression, periplasmic transport, correct folding, and assembly of two different polypeptide chains. Among the different vector formats and arrangements, bicistronic vectors with the first cistron encoding the light chain and the Frontiers in Immunology | B Cell Biology second cistron encoding the Fd fragment are optimal (56). Even aglycosylated full-size IgGs were successfully produced in E. coli (72, 73). In our view, t.....
Document: The Fab format requires expression, periplasmic transport, correct folding, and assembly of two different polypeptide chains. Among the different vector formats and arrangements, bicistronic vectors with the first cistron encoding the light chain and the Frontiers in Immunology | B Cell Biology second cistron encoding the Fd fragment are optimal (56). Even aglycosylated full-size IgGs were successfully produced in E. coli (72, 73). In our view, the raison d'être of complete IgG production in E. coli is doubtful.
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