Author: Frenzel, André; Hust, Michael; Schirrmann, Thomas
Title: Expression of Recombinant Antibodies Document date: 2013_7_29
ID: 06o7pa3d_46
Snippet: Recently, transient production of IgG-like scFv-Fc antibodies in the HEK293-6E cell line, a genetically modified variant with a truncated version of EBNA1 growing in suspension and chemically defined serum-free medium (183, 184) , achieved volumetric yields of up to 0.6 g/L by simple shake flask cultivation. Improved production media, fed-batch supplementation, and well-controlled bioreactor processes allow higher cell densities and prolonged pro.....
Document: Recently, transient production of IgG-like scFv-Fc antibodies in the HEK293-6E cell line, a genetically modified variant with a truncated version of EBNA1 growing in suspension and chemically defined serum-free medium (183, 184) , achieved volumetric yields of up to 0.6 g/L by simple shake flask cultivation. Improved production media, fed-batch supplementation, and well-controlled bioreactor processes allow higher cell densities and prolonged production time, both enhancing the yield. Backliwal and colleagues (177) combined optimized PEI-based transfection at high-cell densities with the coexpression of cell cycle regulators p18 and p21, acidic fibroblast growth factor, valproic acid supplementation, consequent maintenance at high-cell densities of cells/milliliter and up-scaling to 2 L and achieved production levels of more than 1 g IgG within 2 weeks after transient transfection.
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