Author: Liu, Xinsheng; Zhao, Donghong; Zhou, Peng; Zhang, Yongguang; Wang, Yonglu
                    Title: Evaluation of the Efficacy of a Recombinant Adenovirus Expressing the Spike Protein of Porcine Epidemic Diarrhea Virus in Pigs  Document date: 2019_10_8
                    ID: 1lxd2457_22
                    
                    Snippet: 2.9. Viral RNA Extraction and Real-Time PCR. Viral RNA was extracted from intestinal contents using the RNeasy Mini Kit (Qiagen, USA) according to the manufacturer's instructions. Real-time PCR targeting the PEDV N gene was performed as described previously [17] ......
                    
                    
                    
                     
                    
                    
                    
                    
                        
                            
                                Document: 2.9. Viral RNA Extraction and Real-Time PCR. Viral RNA was extracted from intestinal contents using the RNeasy Mini Kit (Qiagen, USA) according to the manufacturer's instructions. Real-time PCR targeting the PEDV N gene was performed as described previously [17] .
 
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