Evaluation of the Efficacy of a Recombinant Adenovirus Expressing the Spike Protein of Porcine Epidemic Diarrhea Virus in Pigs

Liu, Xinsheng; Zhao, Donghong; Zhou, Peng; Zhang, Yongguang; Wang, Yonglu

In recent years, many studies have been conducted on PEDV vaccines, including PEDV inactivated/killed vaccines, attenuated vaccines, and gene-engineering vaccines [14, 16] . However, to date, PEDV commercial vaccines cannot completely prevent and control PEDV infection, so it is still necessary to develop new vaccines and to improve current Each group contained five 4-week-old weaned piglets. b-d Pigs in these three groups were vaccinated intramuscularly with 10 10 PFU/mL Ad-blank, 10 10 PFU/ mL rAd-PEDV-S, and 2 mL of commercial PEDV inactivated vaccine (inactivated virus of the genotype GIIb strain; virus titer before inactivation ≥ 10 7 TCID 50 ), respectively. e Each pig was challenged orally with a virus suspension containing 2 × 10 5 TCID 50 highly virulent genotype GIIb PEDV strain CH/ HNPJ/2017. Dpv: days post vaccination; IM: intramuscularly.